|
| Status |
Public on Jun 14, 2018 |
| Title |
CD8 No2 IL27 |
| Sample type |
RNA |
| |
|
| Source name |
primary CD8 T cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: wild type
|
| Treatment protocol |
Affymetrix GeneChip Mouse Genome 430 2.0 Arrays were used to measure the resulting mRNA levels.
|
| Growth protocol |
Naïve CD4+ and CD8+ T cells were isolated from WT or IL27ra KO mice, and differentiated in vitro with or without IL-27. Cells were collected at 72 hours for CD8+ and 96 hours for CD4+.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
standard Affymetrix protocol
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
Naïve T cells stimulated with IL-27
|
| Data processing |
Individual .CEL files were RMA normalized and merged to an expression matrix using the ExpressionFileCreator of GenePattern with default parameters.
|
| |
|
| Submission date |
Apr 16, 2018 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Asaf Madi |
| E-mail(s) |
asafmadi@gmail.com
|
| Organization name |
BWH
|
| Department |
Neurology
|
| Lab |
Ana Carrizosa Anderson
|
| Street address |
60 Fenwood Road, BTM 10016F
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE113216 |
Microarray expression data of naïve CD4 and CD8 T cells stimulated with IL27. |
| GSE113968 |
The induction and transcriptional regulation of the co-inhibitory gene module in T cells by IL-27 |
|
