|
| Status |
Public on Oct 01, 2008 |
| Title |
oxidative stress, t=0 |
| Sample type |
RNA |
| |
|
| Source name |
time 0 following H2O2 treatment
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain Y262
|
| Treatment protocol |
At time 0, cells were treated with 0.3mM of H2O2 or 0.1% of MMS.
|
| Growth protocol |
Cells were grown in YPD to mid-log phase in 25 degrees.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using MasterPure™ (EPICENTER Biotechnologies).
|
| Label |
biotin
|
| Label protocol |
cDNA was prepared using poly(T) primers, labeled cRNA was prepared using the GeneChip system according to manufacturer instructions.
|
| |
|
| Hybridization protocol |
GeneChip system according to manufacturer instructions.
|
| Scan protocol |
GeneChip system according to manufacturer instructions.
|
| Description |
Gene expression following treatment with 0.3mM of H2O2
|
| Data processing |
RMA algorithms using bioconductor software implemented in R.
|
| |
|
| Submission date |
Jul 23, 2008 |
| Last update date |
Jul 24, 2008 |
| Contact name |
Ophir Shalem |
| E-mail(s) |
ophir.shalem@weizmann.ac.il
|
| Organization name |
Weizmann Inst.
|
| Street address |
Hertzel
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL2529 |
| Series (2) |
| GSE12220 |
Changes in Saccharomyces cerevisiae mRNA abundance following oxidative stress and DNA damage stress |
| GSE12222 |
Transient transcriptional responses to stress are generated by opposing effects of mRNA production and degradation |
|
