|
| Status |
Public on Sep 30, 2010 |
| Title |
wild type DMSO control 3 |
| Sample type |
RNA |
| |
|
| Source name |
yeast
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Saccharomyces cerevisiae strain SEY6210 treated with the corresponding amount of DMSO (used to dissolve OGT2468) in the treatment experiments
|
| Biomaterial provider |
Sabine Strahl's laboratory
|
| Growth protocol |
Mid-log phase cells were grown in the presence of DMSO for 3 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from exponentially growing cells by the “mechanical disruption protocol” using the RNeasy kit (Qiagen, Hilden, Germany), following the instructions of the manufacturer. RNA concentrations were determined by measuring absorbance at 260 nm. RNA purity and integrity were assessed using RNA Nano Labchips in an Agilent 2100B Bioanalyzer (Agilent Technologies, Palo Alto, CA) following the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Following affymetrix labelling instructions
|
| |
|
| Hybridization protocol |
Following affymetrix hybridation instructions
|
| Scan protocol |
Following affymetrix scanning instructions
|
| Description |
All the procedure for microarrays experiments was carried out following the instructions of affymetrix for each of the steps
|
| Data processing |
CEL files from scaner were analyzed using GCRMA algorithm from Bioconductor software
|
| |
|
| Submission date |
Jul 21, 2008 |
| Last update date |
Dec 22, 2009 |
| Contact name |
Javier Arroyo |
| E-mail(s) |
jarroyo@farm.ucm.es, rgarcias@farm.ucm.es
|
| Phone |
0034-913941746
|
| URL |
http://ucm.es/info/mfar
|
| Organization name |
Facultad de Farmacia (UCM)
|
| Department |
Microbiologia II
|
| Lab |
U4-Javier Arroyo
|
| Street address |
Plaza Ramon y Cajal S/N
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
|
| Platform ID |
GPL2529 |
| Series (3) |
| GSE12180 |
Wild type 3 hours exposure to 1 microM OGT2468 |
| GSE12193 |
Wild type 3 hours exposure to 0.1 microM OGT2468 |
| GSE12197 |
O-glycosylation inhibition and O-glycosylation mutants |
|
