|
| Status |
Public on Jul 15, 2008 |
| Title |
EWS primary tumor relapse (R58) |
| Sample type |
RNA |
| |
|
| Source name |
EWS human sample
|
| Organism |
Homo sapiens |
| Characteristics |
EWS primary tumor (REL - relapse)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix One-Cycle protocol from 5 ug total RNA (GeneChip® Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000, Affymetrix.
|
| Description |
Gene expression data from EWS primary tumor
|
| Data processing |
Single samples were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. Data were then rescaled within each array and between arrays through an affine transformation (Bengtsson and Hossjer 2006).
|
| |
|
| Submission date |
Jul 14, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Daniel Remondini |
| E-mail(s) |
daniel.remondini@unibo.it
|
| Organization name |
Bologna University
|
| Department |
Physics and Astronomy - DIFA
|
| Street address |
Viale Berti Pichat 6/2
|
| City |
Bologna |
| ZIP/Postal code |
40127 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE12102 |
Overcoming resistance to conventional drugs in Ewing’s sarcoma and identification of molecular predictors of outcome. |
|
| Relations |
| Reanalyzed by |
GSE119087 |
