|
| Status |
Public on Oct 18, 2008 |
| Title |
Renal Clear Cell Carcinoma_047023 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
mRNA from Renal Clear Cell Carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
Organism Part: Kidney
Disease State: Clear Cell Carcinoma
BioSourceType: Frozen Sample
BioSource Provider: University of North Carolina
|
| Growth protocol |
Frozen RCCs were obtained through University of North Carolina - Chapel Hill's Tissue Procurement Facility
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Quality Control: RNA quality was determined using an Agilent LabChip Bioanalyzer. H&E was performed on tumor tissue samples to confirm clear cell histopathology
RNA was extracted with Qiagen RNeasy kit.
|
| Label |
Cy5
|
| Label protocol |
Tumor sample RNA and reference RNA was labelled by UNC Lineberger Comprehensive Cancer Center Genomics Core
|
| |
|
| Channel 2 |
| Source name |
Perou lab reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
BioSourceType: Cancer cell lines
|
| Growth protocol |
Frozen RCCs were obtained through University of North Carolina - Chapel Hill's Tissue Procurement Facility
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Quality Control: RNA quality was determined using an Agilent LabChip Bioanalyzer. H&E was performed on tumor tissue samples to confirm clear cell histopathology
RNA was extracted with Qiagen RNeasy kit.
|
| Label |
Cy3
|
| Label protocol |
Tumor sample RNA and reference RNA was labelled by UNC Lineberger Comprehensive Cancer Center Genomics Core
|
| |
|
| |
| Hybridization protocol |
Tumor sample RNA and reference RNA were hybridized on Agilent Whole Human Genome 4x44k arrays by UNC Lineberger Comprehensive Cancer Center Genomics Core
|
| Scan protocol |
Agilent gene chips were scanned on an Agilent scanner by UNC Lineberger Comprehensive Cancer Center Genomics Core
|
| Description |
Gene expression data from Human RCC sample
|
| Data processing |
LOWESS normalized, background subtracted VALUE data obtained from log2 of processed Red signal/processed Green signal. UNC Microarry Database software was used.
|
| |
|
| Submission date |
Jul 03, 2008 |
| Last update date |
Oct 18, 2008 |
| Contact name |
Kimryn Rathmell |
| E-mail(s) |
kimryn.rathmell@vanderbilt.edu
|
| Organization name |
Vanderbilt
|
| Department |
Medicine
|
| Lab |
Rathmell
|
| Street address |
2220 Pierce Ave
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE11985 |
Gene expression analysis in renal cell carcinoma |
|
