|
| Status |
Public on Mar 03, 2018 |
| Title |
BIN_UQMoore_OVA_L_2057 |
| Sample type |
SRA |
| |
|
| Source name |
Ovarian tissues left, animal 2057
|
| Organism |
Bos indicus |
| Characteristics |
breed: Brahman
animal id: 2057
puberty status: post-puberty
tissue: Ovarian tissues left
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol and Rneasy mini kit (QIAGEN)
TruSeq RNA sample preparation kit (illumina)
libraries were multiplex per lane and paried-end sequenced, with Illumina HiSeq 2000 analyzer
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Sequencing libraries made using Illumina TrueSeq stranded mRNA Sample Preparation kit
mRNA was purified and converted to double stranded cDNA with adapters and indexes added to each sample
HiSeq 2000 sequencer analyzer (Illumina San Diego, CA) was used on each sample
Sequenced reads were analyzed using CLCBio Genomic workbench software
Quality control was performed using NGS tool
Genome_build: UMD3.1.74
Supplementary_files_format_and_content: Reads were normalized and RPKM was calculated using CLCBio Genomics workbench software for counts data text files
|
| |
|
| Submission date |
Mar 02, 2018 |
| Last update date |
Mar 07, 2018 |
| Contact name |
Marina Fortes |
| E-mail(s) |
m.fortes@uq.edu.au
|
| Organization name |
The University of Queensland
|
| Department |
School of Chemistry and Molecular Biosciences
|
| Lab |
Livestock Genomics
|
| Street address |
SCMB, room 454 bld 76
|
| City |
Brisbane |
| State/province |
Queensland |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platform ID |
GPL20723 |
| Series (1) |
| GSE111339 |
Transcriptome related to pubertal development in Brahman heifers |
|
| Relations |
| SRA |
SRX3757206 |
| BioSample |
SAMEA104618954 |
