|
| Status |
Public on Feb 27, 2019 |
| Title |
STING-/- MEF_pBabe_control |
| Sample type |
RNA |
| |
|
| Source name |
STING-/- MEF cells reconstituted with pBabe vector
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Primary MEF cells
|
| Treatment protocol |
The MEF cells were treated with double strand DNA using Lipofectamine 2000 for 9 hours.
|
| Growth protocol |
STING-/- MEF cells were cultured in DMEM containing 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
300ng of Total RNA per sample was prepared using the GeneChip WT PLUS Reagent Kit (Affymetrix part#902280) and yielded cDNA product for fragmentation and labeling
|
| |
|
| Hybridization protocol |
Fragmented and Labeled products were hybridized to GeneChip Mouse Gene 2.0 ST Arrays (Affymetrix part# 902500). Washing and staining of arrays took place on the GeneChip Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned with GeneChip Scanner 3000 7G system
|
| Description |
STING-/- MEF cells reconstituted with pBabe vector
|
| Data processing |
Raw .CEL files were were analyzed with in the Affymetrix Expression Console software v.1.3.1.187 using rma-sketch normalization with Affymetrix default analysis settings to generate .CHP files.
|
| |
|
| Submission date |
Feb 27, 2018 |
| Last update date |
Feb 27, 2019 |
| Contact name |
Anthony Griswold |
| E-mail(s) |
agriswold@med.miami.edu
|
| Organization name |
University of Miami
|
| Street address |
1501 NW 10th Ave, BRB 318
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE111196 |
Gene expression in STING-/- MEF cells expressing STING R284S variant |
| GSE111198 |
Pro-Inflammation Associated with a Gain-of-Function Mutation (R284S) in the Innate Immune Sensor STING |
|
