|Public on Oct 15, 2008
|Angioimmunoblastic T-cell lymphoma patient (AITL3)
|Human Universal Reference Total RNA (Beckton Dickinson)
|Human Universal Reference Total RNA
|Cy3-labeled cRNA was generated from 0.5 ug of total RNA according to the manufacturer's protocol (Agilent Technology).
|Total RNA from frozen lymph node tissue
|Lymph node from angioimmunoblastic T-cell lymphoma patient (AITL3)
|Frozen lymphoma tissues were ground to powder and lysed in lysis buffer Trizol reagent (Invitrogen).
|Cy5-labeled cRNA was generated from 0.5 ug of total RNA according to the manufacturer's protocol (Agilent Technology).
|The probe consisted of a mixture of Cy5-labeled patient's cRNA and Cy3-labeled control cRNA was hybridized for 17 hours at 65C on the Agilent 44K human oligo microarray.
|Hybridized array slides were scanned at 10-um resolution using an Agilent G2565BA fluorescence dual laser scanner for Cy3 and Cy5 excitation.
|Gene expression data from lymphoma tissue of an angioimmunoblastic T-cell lymphoma patient
|Image intensity data were recorded and transferred into text files using Feature Extraction software, version 9.1.
|Jul 01, 2008
|Last update date
|Oct 15, 2008
|Department of Hematology and Oncology
|Gene expression profiling of peripheral T-cell lymphoma including gamma delta T-cell lymphoma