|
| Status |
Public on Oct 15, 2008 |
| Title |
Hepatosplenic gamma delta T cell lymphoma patient (GD-hs2) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Human Universal Reference Total RNA (Beckton Dickinson)
|
| Organism |
Homo sapiens |
| Characteristics |
Human Universal Reference Total RNA
|
| Biomaterial provider |
Beckton Dickinson
|
| Extracted molecule |
total RNA |
| Extraction protocol |
N/A
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled cRNA was generated from 0.5 ug of total RNA according to the manufacturer's protocol (Agilent Technology).
|
| |
|
| Channel 2 |
| Source name |
Total RNA from frozen lymph node tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Lymph node from hepatosplenic gamma delta T-cell lymphoma patient (GD-hs2)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Frozen lymphoma tissues were ground to powder and lysed in lysis buffer Trizol reagent (Invitrogen).
|
| Label |
Cy5
|
| Label protocol |
Cy5-labeled cRNA was generated from 0.5 ug of total RNA according to the manufacturer's protocol (Agilent Technology).
|
| |
|
| |
| Hybridization protocol |
The probe consisted of a mixture of Cy5-labeled patient's cRNA and Cy3-labeled control cRNA was hybridized for 17 hours at 65C on the Agilent 44K human oligo microarray.
|
| Scan protocol |
Hybridized array slides were scanned at 10-um resolution using an Agilent G2565BA fluorescence dual laser scanner for Cy3 and Cy5 excitation.
|
| Description |
Gene expression data from lymphoma tissue of a hepatosplenic gamma delta T-cell lymphoma patient
|
| Data processing |
Image intensity data were recorded and transferred into text files using Feature Extraction software, version 9.1.
|
| |
|
| Submission date |
Jun 30, 2008 |
| Last update date |
Oct 15, 2008 |
| Contact name |
Kana Miyazaki |
| E-mail(s) |
k-myzk@clin.medic.mie-u.ac.jp
|
| Phone |
81-59-231-5418
|
| Organization name |
Mie University
|
| Department |
Department of Hematology and Oncology
|
| Street address |
2-174 Edobashi
|
| City |
Tsu |
| State/province |
Mie |
| ZIP/Postal code |
514-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE11946 |
Gene expression profiling of peripheral T-cell lymphoma including gamma delta T-cell lymphoma |
|
