|
| Status |
Public on Feb 10, 2018 |
| Title |
Health Children rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Health Children
|
| Organism |
Homo sapiens |
| Characteristics |
disease: healthy
tissue: Peripheral blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
total RNA
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Transcriptome Array 2.0 were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
|
| Description |
Gene expression data from Health Children
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100
|
| |
|
| Submission date |
Feb 09, 2018 |
| Last update date |
Feb 10, 2018 |
| Contact name |
shipeng Li |
| E-mail(s) |
shipengli6@163.com
|
| Phone |
13311046526
|
| Organization name |
Beijing children's hospital
|
| Street address |
Nan Li Shi Road No. 56,Beijing
|
| City |
beijng |
| ZIP/Postal code |
100045 |
| Country |
China |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE110396 |
Expression Profile of Noncoding RNAs and mRNA in Children with Systemic Lupus Erythematosus |
|
