|
| Status |
Public on Nov 30, 2018 |
| Title |
Muscle_pre_10 |
| Sample type |
RNA |
| |
|
| Source name |
vastus lateralis muscle, before HIIT
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: vastus lateralis muscle
gender: male
time point: pre
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from muscle biopsy samples using the miRNeasy® Mini Kit (Qiagen, Hilden, Germany) accoding to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared using GeneChip® WT PLUS Reagent Kit according to the GeneChipR WT PLUS Reagent Kit User Manual from 500 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.5 ug of cDNA were hybridized for 16 hr at 45C on the Human Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G according to the Affymetrix® GeneChip® Command Console® User Manual.
|
| Data processing |
The data were analyzed with Affymetrix® Expression Console™ Software and RMA normalization was conducted.
|
| |
|
| Submission date |
Jan 25, 2018 |
| Last update date |
Nov 30, 2018 |
| Contact name |
Eri Miyamoto-Mikami |
| Organization name |
National Institute of Fitness and Sports in Kanoya
|
| Department |
Department of Sports and Life Science
|
| Street address |
1 Shiromizu
|
| City |
Kanoya |
| State/province |
Kagoshima |
| ZIP/Postal code |
891-2393 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE109657 |
Skeletal muscle response to high-intensity intermittent exercise training in young men |
|
