|
| Status |
Public on Jan 19, 2018 |
| Title |
HepG2 cells with control shRNA, biological replicate 3 [shCt-3] |
| Sample type |
RNA |
| |
|
| Source name |
HepG2_control shRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HepG2
cell type: immortalized hepatocellular carcinoma (HCC) cell line
transfected with: control shRNA
|
| Treatment protocol |
Constructs including MYH10 shRNAs or the control shRNAs were transfected into HepG2 cells separately using Lipofectamine 2000.
|
| Growth protocol |
HepG2 cells were maintained in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; HyClone, USA) supplemented with 10% fetal bovine serum (FBS; HyClone, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin. The cells were incubated at 37°C in a humidified incubator containing 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested from HepG2 cells with treatment using the RNeasy mini kit (QIAGEN, Germany) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were performed by CapitalBio Corporation (Beijing, China) according to the manufacturer’s instructions (Affymetrix, USA).
|
| |
|
| Hybridization protocol |
As per manufacturer (Affymetrix)
|
| Scan protocol |
As per manufacturer (Affymetrix)
|
| Description |
shCt-3
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 18, 2018 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Gangqiao Zhou |
| E-mail(s) |
zhougq114@126.com
|
| Organization name |
Beijing Institute of Radiation Medicine
|
| Street address |
No. 27, Taiping Road, Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE109358 |
Integrative genomic analysis in HCC samples [MYH] |
| GSE109361 |
Integrative genomic analysis in HCC samples |
|
