|
| Status |
Public on Jan 17, 2018 |
| Title |
HFD 2 LKSneg |
| Sample type |
RNA |
| |
|
| Source name |
bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
diet: high fat diet
tissue: bone marrow
gender: male
cell type: BM LKSneg
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted from cells was subjected to nCounter RNA sample preparation according to the manufacturer’s instructions.
|
| Label |
labeled by manufacturer
|
| Label protocol |
Labeled probes are provided by the manufacturer.
|
| |
|
| Hybridization protocol |
100 ng of extracted RNA was used for hybridization to the probes at 65°C for 18 hours, following the manufacturer’s protocol.
|
| Scan protocol |
Hybridized probes were counted on nCounter Prep Station and Digital Analyzer
|
| Data processing |
The nCounter results were analyzed statistically using manufacturer's software nSolver 2.5.
|
| |
|
| Submission date |
Jan 16, 2018 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Lisa Ann Borghesi |
| E-mail(s) |
borghesi@pitt.edu
|
| Phone |
412-383-9737
|
| Organization name |
University of Pittsburgh
|
| Department |
Immunology
|
| Street address |
200 Lothrop ST
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15261 |
| Country |
USA |
| |
|
| Platform ID |
GPL24518 |
| Series (1) |
| GSE109269 |
Bone marrow lympho-myeloid malfunction in obesity requires precursor cell-autonomous TLR4 |
|
