|
| Status |
Public on Jun 17, 2018 |
| Title |
04_1299+Dox 100nM 10759 |
| Sample type |
RNA |
| |
|
| Source name |
cells grown with 0.5ug/ml doxycycline, added 100nM of IACS-10759 for 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H1299
cell type: lung cancer cell line
|
| Treatment protocol |
pINDUCER20-SMARCA4 viral vector stably transduced in H1299 cell lines. Cells were then selected using G418 (0.4mg/ml). SMARCA4 induced using doxycycline (0.5microgram/ml) for 5 days and cells also treated with IACS-10759 (1000nM) for 24 hours prior to extraction
|
| Growth protocol |
H1299 cells were maintained in 10% FBS and pen/strep supplemented RPMI-1640 media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with RNAeasy Kit from Qiagen,Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the kit for Affymetrix arrays
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol applicable to Clariom_S_Human platform
|
| Scan protocol |
Standard Affymetrix scanning protocol
|
| Data processing |
Expression Console
|
| |
|
| Submission date |
Jan 10, 2018 |
| Last update date |
Jun 17, 2018 |
| Contact name |
Andrew Futreal |
| Organization name |
University of Texas MD Anderson Cancer Center
|
| Street address |
1515 Holcombe Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (2) |
| GSE109010 |
Mutations in the SWI/SNF chromatin remodeling complex induce metabolic rewiring and dependence on oxidative phosphorylation [Affymetrix] |
| GSE109011 |
Mutations in the SWI/SNF chromatin remodeling complex induce metabolic rewiring and dependence on oxidative phosphorylation |
|
