|
| Status |
Public on Nov 12, 2020 |
| Title |
Flayed1 5mm flower bud |
| Sample type |
SRA |
| |
|
| Source name |
Flayed1 5mm flower bud
|
| Organism |
Erythranthe lewisii |
| Characteristics |
tissue: 5mm Flower bud
genotype/variation: flayed1 mutant
|
| Treatment protocol |
EMS mutagenesis was performed using the Mimulus lewisii Pursh inbred line LF10 as described (Owen and Bradshaw, 2011). Another inbred line SL9 was used to generate the “flayed x SL9” F2 populations.
|
| Growth protocol |
Plants were grown in the University of Connecticut research greenhouses under natural light supplemented with sodium vapor lamps, ensuring a 16-hr day length.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol (Ambion)
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Small RNAs
|
| Data processing |
Small RNA reads were quality-controled, adaptor-trimmed and collapsed into tag-count format
|
| |
|
| Submission date |
Dec 26, 2017 |
| Last update date |
Nov 12, 2020 |
| Contact name |
Rui Xia |
| E-mail(s) |
rxia@scau.edu.cn
|
| Organization name |
South China Agricultural University
|
| Department |
Horticulture
|
| Lab |
Xia
|
| Street address |
483 Wushan Rd, Tianhe
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510640 |
| Country |
China |
| |
|
| Platform ID |
GPL24433 |
| Series (1) |
| GSE108530 |
Developmental genetics of corolla tube formation in monkeyflowers (Mimulus): role of the tasiRNA-ARF pathway |
|
| Relations |
| BioSample |
SAMN08243399 |
| SRA |
SRX3513371 |
