|
Status |
Public on Nov 30, 2017 |
Title |
T2-HBEC3 200 µg DEP/mL 72 h [200793290121_H] |
Sample type |
RNA |
|
|
Source name |
Human bronchial epithelial cell line
|
Organism |
Homo sapiens |
Characteristics |
agent: 200 microg DEP/mL time point: 72h cell line: T2-HBEC3
|
Treatment protocol |
Cells were exposed to different concentrations of DEP (SRM2975) at different time-points.
|
Growth protocol |
HBEC3 and T2-HBEC3 were maintained in 1:1 mixture of LHC-9 and RPMI1640.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Isol-RNA Lysis Reagent. Quality control was performed with Agilent Bioanalyzer.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the TargetAmp-Nano Labelig Kit for Illumina arrays
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|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Illumina iScan System
|
Description |
Replicate 1 SAMPLE 56
|
Data processing |
Bead level data were summarized by Illumina GenomeStudio Software v2011.1 Bead summary data was normalized using the quantile method in the Lumi package (R). Only probes with a detection p-value < 0.01 in more than 50 % of the arrays were included for further analyses.
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|
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Submission date |
Nov 29, 2017 |
Last update date |
Nov 30, 2017 |
Contact name |
Steen Mollerup |
E-mail(s) |
steen.mollerup@stami.no
|
Phone |
0047 23195297
|
Organization name |
National Institute of Occupational Health
|
Department |
Department of Chemical and Biological Work Environment
|
Lab |
Section for Toxicological and Biological Work Environment
|
Street address |
Gydas vei, 8
|
City |
OSLO |
ZIP/Postal code |
0363 |
Country |
Norway |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE107481 |
In vitro transformation of human bronchial epithelial cells by diesel exhaust particles |
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