|
| Status |
Public on Nov 30, 2017 |
| Title |
T2-HBEC3 200 µg DEP/mL 72 h [200793290121_H] |
| Sample type |
RNA |
| |
|
| Source name |
Human bronchial epithelial cell line
|
| Organism |
Homo sapiens |
| Characteristics |
agent: 200 microg DEP/mL
time point: 72h
cell line: T2-HBEC3
|
| Treatment protocol |
Cells were exposed to different concentrations of DEP (SRM2975) at different time-points.
|
| Growth protocol |
HBEC3 and T2-HBEC3 were maintained in 1:1 mixture of LHC-9 and RPMI1640.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Isol-RNA Lysis Reagent. Quality control was performed with Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the TargetAmp-Nano Labelig Kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Illumina iScan System
|
| Description |
Replicate 1
SAMPLE 56
|
| Data processing |
Bead level data were summarized by Illumina GenomeStudio Software v2011.1 Bead summary data was normalized using the quantile method in the Lumi package (R). Only probes with a detection p-value < 0.01 in more than 50 % of the arrays were included for further analyses.
|
| |
|
| Submission date |
Nov 29, 2017 |
| Last update date |
Nov 30, 2017 |
| Contact name |
Steen Mollerup |
| E-mail(s) |
steen.mollerup@stami.no
|
| Phone |
0047 23195297
|
| Organization name |
National Institute of Occupational Health
|
| Department |
Department of Chemical and Biological Work Environment
|
| Lab |
Section for Toxicological and Biological Work Environment
|
| Street address |
Gydas vei, 8
|
| City |
OSLO |
| ZIP/Postal code |
0363 |
| Country |
Norway |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE107481 |
In vitro transformation of human bronchial epithelial cells by diesel exhaust particles |
|
