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Sample GSM2858098 Query DataSets for GSM2858098
Status Public on Nov 30, 2020
Title m21_L_H3K4Me3-1
Sample type SRA
 
Source name Rat brain tissues
Organism Rattus norvegicus
Characteristics tissue: ipsilateral brain
age: 21 mon old
agent: none
chip antibody: H3K4Me3(Millipore,17-614)
disease: intracerebral hemorrhage
Extracted molecule genomic DNA
Extraction protocol Lysates were clarified from sonicated cell and histone-DNA complexes were isolated with antibody.
he libraries were prepared following the manufacturer's instructions (ThruPLEX DNA-seq 48S Kit, R400427)and applied to Illumina NextSeq 500 system for 150nt pair-end sequencing by ABlife Inc.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NextSeq 500
 
Description the ipsilateral brain tissue of intracerebral hemorrhage rats
Data processing Illumina Casava1.9 software used for basecalling.
3' adapter (end1:AGATCGGAAGAGC;end2:AGATCGGAAGAGC) trimmed with cutadapt
Reads were mapped using bowtie2 -N 1. Uniq mapped reads were remained.
macs14 was used to peakcalling with default parameters
homer was used to find motifs with options -len 5,6,7,8,9,10,11,12
Genome_build: Rattus norvegicus 6.0
Supplementary_files_format_and_content: peaks
 
Submission date Nov 15, 2017
Last update date Nov 30, 2020
Contact name dong chen
Organization name ablife
Street address GaoXin 2nd Road
City wuhan
State/province Hubei
ZIP/Postal code 430064
Country China
 
Platform ID GPL20084
Series (2)
GSE106951 High-throughput sequencing and analysis of gene modification regulated by epigenetics modification in adult and old intracerebral hemorrhage rats. [ChIP-Seq]
GSE106952 High-throughput sequencing and analysis of gene modification regulated by epigenetics modification in adult and old intracerebral hemorrhage rats.
Relations
BioSample SAMN08031709
SRA SRX3398096

Supplementary data files not provided
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

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