|
| Status |
Public on Apr 24, 2008 |
| Title |
HA1ER M2 rep3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Glucose starved, MondoA knockdown HA1ER cells
|
| Organism |
Homo sapiens |
| Characteristics |
Glucose starved
|
| Treatment protocol |
Cell were plated on 60mm dishes at 80% confluence, glucose was removed overnight followed by a 3 hour add-back of 2-deoxyglucose
|
| Growth protocol |
Cells were maintained in alpha-MEM with 10% Bovine Growth Serum and 0.5% Pen/Strep
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was produced by RNAeasy with DNase treatment.
|
| Label |
Cy5
|
| Label protocol |
Agilent two-color LRILAK labeling protocol
|
| |
|
| Channel 2 |
| Source name |
2-deoxyglucose induced, MondoA knockdown HA1ER cells
|
| Organism |
Homo sapiens |
| Characteristics |
2-deoxyglucose induced
|
| Treatment protocol |
Cell were plated on 60mm dishes at 80% confluence, glucose was removed overnight followed by a 3 hour add-back of 2-deoxyglucose
|
| Growth protocol |
Cells were maintained in alpha-MEM with 10% Bovine Growth Serum and 0.5% Pen/Strep
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was produced by RNAeasy with DNase treatment.
|
| Label |
Cy3
|
| Label protocol |
Agilent two-color LRILAK labeling protocol
|
| |
|
| |
| Hybridization protocol |
Agilent two-color gene expression hyb/wash protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505B Microarray Scanner at 5 micron resolution. Images were quantified using Agilent Feature Extraction software version 9.5
|
| Description |
HA1ER M2 rep3
|
| Data processing |
LOWESS normalized data obtained from log2 of red processed signal/processed green signal
|
| |
|
| Submission date |
Apr 23, 2008 |
| Last update date |
Apr 24, 2008 |
| Contact name |
Donald Ayer |
| E-mail(s) |
don.ayer@hci.utah.edu
|
| Phone |
801-581-5597
|
| Fax |
801-585-6410
|
| URL |
http://www.hci.utah.edu/group/ayer/ayerLab.jsp
|
| Organization name |
University of Utah, Huntsman Cancer Institute
|
| Department |
Oncological Sciences
|
| Lab |
Ayer Lab
|
| Street address |
2000 Circle of Hope
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE11242 |
2-Deoxyglucose induced transcription in control and MondoA knockdown cells |
|
