|
| Status |
Public on Oct 24, 2018 |
| Title |
IMA.sample_10531.47_female |
| Sample type |
SRA |
| |
|
| Source name |
Internal thoracic artery
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD90+ sort
tissue: Internal thoracic artery
age: 47
gender: female
coronary artery disease status: case
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from freshly sorted live DAPI-CD90+ aorta or ITA cells using an RNeasy Micro Kit (Qiagen). All samples had a 260/280 ratio greater than 1.8.
Amplified cDNA was prepared from total RNA using the Ovation RNA-Seq System V2 Kit (NuGEN, San Carlos, CA) according to manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
CAD for CABG
|
| Data processing |
Sequencing reads were aligned to the human reference genome with the STAR aligner using default parameters.
GENCODE v.24 annotations were downloaded and exons were merged on a per gene basis. Reads were counted on merged exons using the HTSeq-count program.
Genome_build: GRCh38
Supplementary_files_format_and_content: tab-delimited file with read counts for all samples
|
| |
|
| Submission date |
Oct 24, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jason C Kovacic |
| E-mail(s) |
jason.kovacic@mountsinai.org
|
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Street address |
1 Gustave L. Levy Place, Box: 1030
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE106116 |
CD90 Identifies Adventitial Mesenchymal Progenitor Cells in Adult Humans |
|
| Relations |
| BioSample |
SAMN07830338 |
| SRA |
SRX3318159 |
