|
| Status |
Public on Feb 15, 2018 |
| Title |
RootHair_gtl1_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Root hair cells
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype: gtl1-1
gfp flourescent: FACS using pEXPA7:NLS-GFP
|
| Treatment protocol |
Seedling roots, 5 days after germination
|
| Growth protocol |
On MS media for 5 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Plotoplasts were isolated from root tissues and GFP-expressing cells were collected by FACS methods. Total RNA from plotoplasts was purified by Qiagen Rneasy kit.
|
| Label |
biotin
|
| Label protocol |
Affy two-cycle labeling (raw 21-26) and one-cycle (everything else) labeling protocol
|
| |
|
| Hybridization protocol |
Standard Operating Procedure performed at the Duke Microarray Facility
|
| Scan protocol |
Standard Operating Procedure performed at the Duke Microarray Facility
|
| Description |
Gene expression data from root hair cells isolated by FACS using pEXPA7:NLS-GFP
|
| Data processing |
The gcRMA implementation in the Bioconductor and R software packages were used for background correction, quantile normalization and expression-estimate computation.
|
| |
|
| Submission date |
Sep 15, 2017 |
| Last update date |
Feb 15, 2018 |
| Contact name |
Michitaro Shibata |
| E-mail(s) |
michitaro.shibata@riken.jp
|
| Organization name |
RIKEN
|
| Department |
CSRS
|
| Lab |
Cell function research team
|
| Street address |
1-7-22 Suehirocho Tsurumi
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE103917 |
Root hair specific transcriptome analysis of gain-of-function and loss-of-function of GTL1 and DF1 |
|
