|
| Status |
Public on Sep 17, 2017 |
| Title |
A549 Low attachment+LA717 7days 1 |
| Sample type |
RNA |
| |
|
| Source name |
A549 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: A549
condition: ow-attachment plate with LA717 containing medium
|
| Treatment protocol |
A549 cells were cultured for 7 days in attachment or low-attachment 96 well plates with 10 % FBS/DMEM supplemented with LA717 (0.03 w/v%) or Milli-Q water.
|
| Growth protocol |
A549 cells were cultured in DMEM supplemented with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed with Quiagen RNeasy mini kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 2.0ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS3000 scanner.
|
| Description |
Gene expression data from A549 cells cultured in 3D condition.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
|
| |
|
| Submission date |
Sep 13, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Natsuki Abe |
| Organization name |
NISSAN CHEMICAL INDUSTORIES, LTD
|
| Street address |
Shiraoka 1470
|
| City |
Shiraoka-shi |
| State/province |
Saitama |
| ZIP/Postal code |
349-0294 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE103845 |
Gene expression profile of A549 cells cultured under a new 3D culture conditions |
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