|
| Status |
Public on Nov 06, 2017 |
| Title |
231_1a3_dac_ra_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
231_1a3_dac_ra
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
genotype/variation: MSCV ALDH1A3 overexpression
atra tx: 100 nM
decitabine tx: 1 uM
replicate: 2
|
| Growth protocol |
All cells grown in DMEM with 10% FBS, 1x antibiotic-antimycotic, 0.25 ug/mL puromycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted with Trizol reagent and PureLink RNA kit (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
CHP files derived from Transcriptome Analysis Console
|
| Data processing |
affymetrix-algorithm-name = rma-gene-full
affymetrix-algorithm-version = 1.0
affymetrix-array-type = HuGene-2_0-st
program-name = Expression Console
program-version = 1.3.1.187
|
| |
|
| Submission date |
Sep 04, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Paola Marcato |
| Organization name |
Dalhousie University
|
| Department |
Pathology
|
| Street address |
PO Box 15000
|
| City |
Halifax |
| State/province |
Nova Scotia |
| ZIP/Postal code |
B3H 4R2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16686 |
| Series (2) |
| GSE103426 |
Expression profiling of MDA-MB-231 and MDA-MB-468 after ALDH1A3 manipulation, all-trans retinoic acid treatment, decitabine treatment |
| GSE103427 |
Profiling of the transcriptional response to all-trans retinoic acid in breast cancer cells reveals RARE-independent mechanisms of gene expression |
|
