|
| Status |
Public on Sep 21, 2010 |
| Title |
Affymetrix expression in med15 |
| Sample type |
RNA |
| |
|
| Source name |
delta-med15
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
Strain: delta-med15. Cells were grown to mid-log phase in rich medium.
|
| Biomaterial provider |
Paulina Wanrooij
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from mid-log phase cultures according to Xue et al. 2004 (Yeast 21(1):25-39).
|
| Label |
biotin
|
| Label protocol |
Labeling was performed according to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
|
| |
|
| Hybridization protocol |
According to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
|
| Scan protocol |
According to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
|
| Description |
Gene expression in the delta-med15 S. pombe strain was analysed using the Affymetrix GeneChip Yeast Genome 2.0 array.
|
| Data processing |
After the ExpressionStat algorithm, data was imported into Genespring GX (Agilent technologies), per-gene normalized to median and filtered on flags.
|
| |
|
| Submission date |
Mar 10, 2008 |
| Last update date |
Sep 21, 2010 |
| Contact name |
Claes M Gustafsson |
| E-mail(s) |
claes.gustafsson@medkem.gu.se
|
| Organization name |
Gothenburg University
|
| Department |
Department of Medical biochemistry and cell biology
|
| Street address |
Medcinaregatan 9A
|
| City |
Gothenburg |
| ZIP/Postal code |
405 30 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE10079 |
A Med15 - Hrp1 complex associates with fission yeast Mediator |
|
