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Sample GSM271922 Query DataSets for GSM271922
Status Public on Sep 21, 2010
Title Affymetrix expression in hrp1 2
Sample type RNA
 
Source name delta-hrp1
Organism Schizosaccharomyces pombe
Characteristics Strain: delta-hrp1. Cells were grown to mid-log phase in rich medium.
Biomaterial provider Paulina Wanrooij
Extracted molecule total RNA
Extraction protocol RNA was isolated from mid-log phase cultures according to Xue et al. 2004 (Yeast 21(1):25-39).
Label biotin
Label protocol Labeling was performed according to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
 
Hybridization protocol According to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
Scan protocol According to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
Description Gene expression in the delta-hrp1 S. pombe strain was analysed using the Affymetrix GeneChip Yeast Genome 2.0 array.
Data processing After the ExpressionStat algorithm, data was imported into Genespring GX (Agilent technologies), per-gene normalized to median and filtered on flags.
 
Submission date Mar 10, 2008
Last update date Sep 21, 2010
Contact name Claes M Gustafsson
E-mail(s) claes.gustafsson@medkem.gu.se
Organization name Gothenburg University
Department Department of Medical biochemistry and cell biology
Street address Medcinaregatan 9A
City Gothenburg
ZIP/Postal code 405 30
Country Sweden
 
Platform ID GPL2529
Series (1)
GSE10079 A Med15 - Hrp1 complex associates with fission yeast Mediator

Data table header descriptions
ID_REF
VALUE Signal value
ABS_CALL absence call
DETECTION P-VALUE the p-value for detection

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 21.1515 P 0.000169227
AFFX-BioB-M_at 36.9693 P 0.000195116
AFFX-BioB-3_at 21.8359 P 6.02111e-05
AFFX-BioC-5_at 54.0937 P 4.42873e-05
AFFX-BioC-3_at 70.7091 P 5.16732e-05
AFFX-BioDn-5_at 135.46 P 4.42873e-05
AFFX-BioDn-3_at 290.795 P 4.42873e-05
AFFX-CreX-5_at 778.576 P 5.16732e-05
AFFX-CreX-3_at 1038.71 P 4.42873e-05
AFFX-DapX-5_at 6.9559 P 0.000581214
AFFX-DapX-M_at 23.0353 P 0.000509415
AFFX-DapX-3_at 21.8484 P 0.000340305
AFFX-LysX-5_at 1.66926 A 0.139482
AFFX-LysX-M_at 0.991905 A 0.52976
AFFX-LysX-3_at 5.71064 P 0.0103167
AFFX-PheX-5_at 0.870567 A 0.60308
AFFX-PheX-M_at 3.45387 A 0.0676614
AFFX-PheX-3_at 3.92664 A 0.300606
AFFX-ThrX-5_at 2.53657 A 0.123572
AFFX-ThrX-M_at 5.55689 P 0.00141043

Total number of rows: 10928

Table truncated, full table size 337 Kbytes.




Supplementary file Size Download File type/resource
GSM271922.CEL.gz 1.0 Mb (ftp)(http) CEL
GSM271922.CHP.gz 56.7 Kb (ftp)(http) CHP
Processed data included within Sample table

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