|
| Status |
Public on Sep 30, 2008 |
| Title |
U133A_626A |
| Sample type |
RNA |
| |
|
| Source name |
U133A, 626A, mixed GCT (Yolk Sac, Embryonal Carcinoma)
|
| Organism |
Homo sapiens |
| Characteristics |
mixed GCT (Yolk Sac, Embryonal Carcinoma)
male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Frozen tissue from a germ cell tumor was minced and homogenized in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
5-12 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Adult Male Germ Cell Tumor from retroperitoneal lymph node metastasis (from same patient as 231B)
|
| Data processing |
RMA (robust multi-array) with median adjustment to match training set
|
| |
|
| Submission date |
Mar 07, 2008 |
| Last update date |
Jul 23, 2008 |
| Contact name |
James Korkola |
| E-mail(s) |
korkolaj@mskcc.org
|
| Phone |
212-639-8281
|
| Organization name |
Memorial Sloan-Kettering
|
| Department |
Cell Biology
|
| Lab |
Chaganti
|
| Street address |
1275 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE10783 |
Validation Set for Prediction of Outcome in Adult Male Germ Cell Tumors |
|
