|
Status |
Public on Sep 30, 2008 |
Title |
U133A_606A |
Sample type |
RNA |
|
|
Source name |
U133A, 606A, mixed GCT (Yolk Sac, Embryonal Carcinoma, Teratoma)
|
Organism |
Homo sapiens |
Characteristics |
mixed GCT (Yolk Sac, Embryonal Carcinoma, Teratoma) male
|
Extracted molecule |
total RNA |
Extraction protocol |
Frozen tissue from a germ cell tumor was minced and homogenized in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
|
Label |
biotin
|
Label protocol |
5-12 µg of total RNA was processed to produce biotinylated cRNA targets.
|
|
|
Hybridization protocol |
standard Affymetrix procedures
|
Scan protocol |
standard Affymetrix procedures
|
Description |
Adult Male Germ Cell Tumor
|
Data processing |
RMA (robust multi-array) with median adjustment to match training set
|
|
|
Submission date |
Mar 07, 2008 |
Last update date |
Jul 23, 2008 |
Contact name |
James Korkola |
E-mail(s) |
korkolaj@mskcc.org
|
Phone |
212-639-8281
|
Organization name |
Memorial Sloan-Kettering
|
Department |
Cell Biology
|
Lab |
Chaganti
|
Street address |
1275 York Ave
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10021 |
Country |
USA |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE10783 |
Validation Set for Prediction of Outcome in Adult Male Germ Cell Tumors |
|