NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM270187 Query DataSets for GSM270187
Status Public on May 12, 2008
Title CE5
Sample type SRA
 
Source name 5 day embryo
Organism Gallus gallus
Characteristics breed: Ross-308™, Tissue:whole embryo, Stage:5 days of incubation
Growth protocol Fertilized Ross-308 eggs were incubated in a Multiquip incubator at 37.5 C with rotation every 6 hours. Chick embryos were collected at day 5, 7, and 9 of incubation to represent the embryonic developmental stages 25-27, 30-31, and 35 respectively (Hamburger and Hamilton 1992). After removal of the amnion embryos were rinsed in 1xPBS, and immediately processed for RNA isolation.
Extracted molecule total RNA
Extraction protocol Low molecular weight RNA was extracted using mirVana Isolation Kit (Ambion) folowing the manufacturer's protocol.
 
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina Genome Analyzer
 
Description 5 day embryo
Data processing Refer to Supplemental Methods of the related manuscript for detail.
 
Submission date Mar 02, 2008
Last update date May 15, 2019
Contact name Evgeny Glazov
E-mail(s) e.glazov@uq.edu.au
Organization name University of Queensland
Department Diamantina Institute
Street address Ipswich Road
City Brisbane
ZIP/Postal code 4102
Country Australia
 
Platform ID GPL10223
Series (1)
GSE10686 Deep sequencing of small RNA libraries from chicken embryo
Relations
SRA SRX029063
BioSample SAMN00116356

Data table header descriptions
SEQUENCE
COUNT count

Data table
SEQUENCE COUNT
TGGAATGTAAAGAAGTATGTATTCGTATGCCGT 260135
TGAGGTAGTAGGTTGTATAGTTTCGTATGCCGT 213816
TGAGGTAGTAGGTTGTATGGTTTCGTATGCCGT 151369
TGAGGTAGTAGATTGTATAGTTTCGTATGCCGT 115834
ACAGTAGTCTGCACATTGGTTATCGTATGCCGT 108066
AGCAGCATTGTACAGGGCTATGATCGTATGCCG 86571
TGGAATGTAAGGAAGTGTGTGGTCGTATGCCGT 69513
TGGAGTGTGACAATGGTGTTTGTCGTATGCCGT 50634
ACAGTAGTCTGCACATTGGTTTCGTATGCCGTC 48601
TCTTTGGTTATCTAGCTGTATGATCGTATGCCG 45918
CAGGCTGGTTAGATGGTTGTCTTCGTATGCCGT 40744
TTAAGACTTGTAGTGATGTTTATCGTATGCCGT 37324
TCACAGTGAACCGGTCTCTTTTCGTATGCCGTC 35667
AATTGCACGGTATCCATCTGTATCGTATGCCGT 34836
AGCAGCATTGTACAGGGCTATCATCGTATGCCG 31107
AACATTCATTGCTGTCGGTGGGTTTCGTATGCC 30241
GAGGAAGAAGGAATATTTTTCGTATGCCGTCTT 28698
TGAGGTAGTAGTTTGTACAGTTTCGTATGCCGT 26819
AACTCTTAGCGGTGGATCACTCGTCGTATGCCG 25285
TACCCTGTAGATCCGAATTTGTTCGTATGCCGT 25054

Total number of rows: 329334

Table truncated, full table size 11586 Kbytes.




Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data included within Sample table
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap