|
| Status |
Public on Jul 12, 2017 |
| Title |
il7r-/- larvae at 4dpf rep1 |
| Sample type |
RNA |
| |
|
| Source name |
il7r-/- zebrafish larvae
|
| Organism |
Danio rerio |
| Characteristics |
tissue: larvae
age: 4 days post hybridization (dpf)
genotype: il7r-/-
|
| Treatment protocol |
We generated il7r-/- homozygous mutant by clustered regularly interspaced short palindromic repeats (CRISPR) / Cas9 technology. Tu and il7r-/- larvae at 4dpf were collected and rinsed with E3 medium . They were put in 1.5 mL RNase tube after discarding excess water and stored in liquid nitrogen.
|
| Growth protocol |
Zebrafish larvae were raised at 28.5°C under a 10/14h dark/light cycle . Embryos were collected after natural spawning and staged developmentally by hours post fertilization (hpf) or days post fertilization (dpf).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on on Zebrafish Gene 1.0 ST Array. GeneChips were washed and stained in GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip2 Scanner 30007G (Affymetrix).
|
| Description |
Gene expression data from il7r-/- larvae at 4dpf
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
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| |
|
| Submission date |
Jul 11, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
shijiao cai |
| E-mail(s) |
shijiaocai0917@tmu.edu.cn
|
| Phone |
15122857061
|
| Organization name |
Tianjin Medical University
|
| Street address |
Qixiangtai road
|
| City |
tianjin |
| ZIP/Postal code |
300070 |
| Country |
China |
| |
|
| Platform ID |
GPL16933 |
| Series (1) |
| GSE101138 |
Expression data from Zebrafish larvae at 4dpf |
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