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Sample GSM2671788 Query DataSets for GSM2671788
Status Public on Jun 17, 2017
Title ERaUtcKO E2 2h-3
Sample type RNA
 
Source name ovariectomized ERaUtcKO uterus, E2 treated for 2h, replicate 3
Organism Mus musculus
Characteristics strain: C57Bl6
genotype/variation: Esr1f/f;PgrCre+
tissue: uterus
Treatment protocol Ovariectomized mice were injected intraperitoneally with vehicle (100 ul saline) , E2 (250 ng in 100 ul saline) or IGF1 (50 ug in 100 ul PBS), or had an osmotic pump (Alza 100 3D; 3 day) containg IGF1 (0.5 mg/ml 0.1N acetic acid) inserted intraperitoneally. Uterine tissue was collected after 2 hours or 24 hours, frozen in liquid nitrogen and crushed
Extracted molecule total RNA
Extraction protocol RNA was isolated using Trizol reagent (Thermo Scientific). RNA was further purified using Qiagen RNAeasy RNA clean-up protocol with DNAse treatment
Label biotin
Label protocol Three hundred nanograms (300 ng) of total RNA were amplified and labeled as directed using the Affymetrix WT Plus Reagent Kit (WT Plus Kit).
 
Hybridization protocol Five and one half micrograms (5.5 μg) of amplified biotin-cDNAs were fragmented and hybridized to each array for 16 hours at 45°C in a rotating hybridization oven. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0001.
Scan protocol Arrays were scanned in an Affymetrix Scanner 3000.
Description gene expression after E2, 2h treatment
Esr1f/f;PgrCre+ mouse on C57B6
Data processing Data was obtained using the GeneChip® Command Console software.
 
Submission date Jun 16, 2017
Last update date Jan 23, 2018
Contact name NIEHS Microarray Core
E-mail(s) microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL20775
Series (1)
GSE100131 Role of ERα in Mediating Female Uterine Transcriptional Responses to IGF1

Data table header descriptions
ID_REF
VALUE log2-rma

Data table
ID_REF VALUE
AFFX-BkGr-GC03_st 6.41429
AFFX-BkGr-GC04_st 5.525
AFFX-BkGr-GC05_st 5.25315
AFFX-BkGr-GC06_st 4.71265
AFFX-BkGr-GC07_st 4.23482
AFFX-BkGr-GC08_st 3.96
AFFX-BkGr-GC09_st 3.66413
AFFX-BkGr-GC10_st 3.4966
AFFX-BkGr-GC11_st 3.25184
AFFX-BkGr-GC12_st 3.09771
AFFX-BkGr-GC13_st 2.90712
AFFX-BkGr-GC14_st 2.86394
AFFX-BkGr-GC15_st 2.82126
AFFX-BkGr-GC16_st 2.83412
AFFX-BkGr-GC17_st 2.91019
AFFX-BkGr-GC18_st 3.03409
AFFX-BkGr-GC19_st 3.18405
AFFX-BkGr-GC20_st 3.32655
AFFX-BkGr-GC21_st 3.65314
AFFX-BkGr-GC22_st 4.50306

Total number of rows: 72688

Table truncated, full table size 1859 Kbytes.




Supplementary file Size Download File type/resource
GSM2671788_16940_935_cKO_E2_2h_Rep_3_MTA-1_0_.CEL.gz 25.0 Mb (ftp)(http) CEL
GSM2671788_16940_935_cKO_E2_2h_Rep_3_MTA-1_0_.sst-rma-gene-full.chp.gz 484.1 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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