|
| Status |
Public on Jun 17, 2017 |
| Title |
WT (Ex3aERKO) E2 24h-3 |
| Sample type |
RNA |
| |
|
| Source name |
ovariectomized WT (Ex3aERKO littermate) uterus, E2 treated for 24h, replicate 3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6
genotype/variation: wildtype
tissue: uterus
|
| Treatment protocol |
Ovariectomized mice were injected intraperitoneally with vehicle (100 ul saline) , E2 (250 ng in 100 ul saline) or IGF1 (50 ug in 100 ul PBS), or had an osmotic pump (Alza 100 3D; 3 day) containg IGF1 (0.5 mg/ml 0.1N acetic acid) inserted intraperitoneally. Uterine tissue was collected after 2 hours or 24 hours, frozen in liquid nitrogen and crushed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol reagent (Thermo Scientific). RNA was further purified using Qiagen RNAeasy RNA clean-up protocol with DNAse treatment
|
| Label |
biotin
|
| Label protocol |
Three hundred nanograms (300 ng) of total RNA were amplified and labeled as directed using the Affymetrix WT Plus Reagent Kit (WT Plus Kit).
|
| |
|
| Hybridization protocol |
Five and one half micrograms (5.5 μg) of amplified biotin-cDNAs were fragmented and hybridized to each array for 16 hours at 45°C in a rotating hybridization oven. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0001.
|
| Scan protocol |
Arrays were scanned in an Affymetrix Scanner 3000.
|
| Description |
gene expression after E2, 24h treatment
WT littermate of Ex3aERKO (Esr1-null) mouse on C57Bl6
|
| Data processing |
Data was obtained using the GeneChip® Command Console software.
|
| |
|
| Submission date |
Jun 16, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE100131 |
Role of ERα in Mediating Female Uterine Transcriptional Responses to IGF1 |
|
