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| Status |
Public on May 31, 2017 |
| Title |
ExVivoLV_DM_rep2_miRNA |
| Sample type |
RNA |
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| Source name |
Left ventricular tissue of DM rat, replicate 2
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| Organism |
Rattus norvegicus |
| Characteristics |
strain: OLETF
disease state: diabetes
tissue: heart
tissue compartment: left ventricle
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA of the rat heart was isolated using a mirVana miRNA isolation kit (Ambion, Austin, TX) and was reverse-transcribed using the miRNA reverse transcription kit in combination with stem-loop Megaplex primer pool (Applied Biosystems, Foster City, CA).
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| Label |
SYBR Green
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| Label protocol |
The PCR was run in a 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA), and miRNA expression was calculated by comparative delta Ct analysis. U6 snRNA (Applied Biosystems, Foster City, CA) was used as an endogenous control.
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| Hybridization protocol |
n/a
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| Scan protocol |
n/a
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| Description |
Diabetes
Sample name: OLETF2
micro RNA
Cardiac cells
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| Data processing |
The normalization and all the data analysis were performed according to the manufacturers instructions.
For the normalization it uses the value of U6, a housekeeping gene.
Target miRNA signals normalized to the housekeeping genes 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)]
The web-based software package automatically performs all deltadeltaCt based fold-change calculations from the uploaded raw threshold cycle data.
normalized data file reports normalized signal (against housekeeping genes).
Fold Change file reports test/control (i.e., Diabetes/Non-diabetes) ratios.
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| Submission date |
May 30, 2017 |
| Last update date |
May 31, 2017 |
| Contact name |
Masaya Tanno |
| E-mail(s) |
tannom@sapmed.ac.jp
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| Phone |
+81116112111
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| Organization name |
Sapporo Medical University
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| Department |
Department of Cardiovascular, Renal and Metabolic Medicine
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| Street address |
S1, W16
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| City |
Sapporo |
| ZIP/Postal code |
060-8543 |
| Country |
Japan |
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| Platform ID |
GPL22820 |
| Series (2) |
| GSE99410 |
Micro RNA array analysis in OLETF, a type 2 diabetic rats, and LETO, a non-diabetic control rats |
| GSE99411 |
Gene expression analysis in OLETF, a type 2 diabetic rats, and LETO, a non-diabetic control rats |
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| Supplementary data files not provided |
| Processed data are available on Series record |
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