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Sample GSM2641108 Query DataSets for GSM2641108
Status Public on Aug 12, 2017
Title AB81, hypoxia, HIF2kd, a
Sample type RNA
 
Source name conditionally immortalized human podocytes (AB81)
Organism Homo sapiens
Characteristics condition: hypoxia
genotype: HIF2kd
Treatment protocol Differentiated human podocytes were treated with hypoxia (1% O2) for 24h.
Growth protocol Conditionally immortalized human podocytes were cultured under permissive conditions (33°C) in RPMI-1640 supplemented with 10% FBS, penicillin (100 U/ml)/streptomycin (100 µg/ml), and 1% ITS. Differentiation was induced by thermoshift to 37°C.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Rneasy kit (Qiagen) following manufacturer's protocol
Label biotin
Label protocol Fragmentation and biotin labelling of each amplified cDNA was processed with the NuGen Encore Biotin Module (NuGen Inc.) and according to manufacturer's protocol.
 
Hybridization protocol standard Affymetrix protocol
Scan protocol GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
Description Gene expression data from conditionally immortalized podocytes (AB81)
Data processing The raw data was normalized using Robust Multichip Algorithm (RMA) using CustomCDF v 18 ENTREZG
 
Submission date May 25, 2017
Last update date Jan 23, 2018
Contact name Felix Eichinger
E-mail(s) eichinge@umich.edu
Organization name The University of Michigan
Department Division of Nephrology
Street address 1150 W. Medical Center Drive
City Ann Arbor
State/province Michigan
ZIP/Postal code 48109
Country USA
 
Platform ID GPL19109
Series (2)
GSE99323 Transcriptome-based network analysis reveals renal cell type-specific dysregulation of hypoxia-associated transcripts [AB81]
GSE99340 Transcriptome-based network analysis reveals renal cell type-specific dysregulation of hypoxia-associated transcripts

Data table header descriptions
ID_REF
VALUE Entrez Gene IDs and Log base 2 expression values

Data table
ID_REF VALUE
1_at 4.369103263
10_at 5.620438255
100_at 9.072674237
1000_at 9.707735202
10000_at 7.907690878
100009676_at 5.949601501
10001_at 8.638426921
10002_at 4.335888131
10003_at 6.079553827
10004_at 3.4273902
100048912_at 2.813308872
100049716_at 6.026832236
10005_at 6.180833226
10006_at 9.709511684
10007_at 11.13408774
10008_at 2.772727263
10009_at 7.266219358
100093630_at 9.535071728
100093698_at 2.360429796
1001_at 5.211101767

Total number of rows: 19612

Table truncated, full table size 403 Kbytes.




Supplementary file Size Download File type/resource
GSM2641108_AB81_H1_HIF2kd_a.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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