|
| Status |
Public on Aug 12, 2017 |
| Title |
AB81 hypoxia, HIF1kd, a |
| Sample type |
RNA |
| |
|
| Source name |
conditionally immortalized human podocytes (AB81)
|
| Organism |
Homo sapiens |
| Characteristics |
condition: hypoxia
genotype: HIF1kd
|
| Treatment protocol |
Differentiated human podocytes were treated with hypoxia (1% O2) for 24h.
|
| Growth protocol |
Conditionally immortalized human podocytes were cultured under permissive conditions (33°C) in RPMI-1640 supplemented with 10% FBS, penicillin (100 U/ml)/streptomycin (100 µg/ml), and 1% ITS. Differentiation was induced by thermoshift to 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Rneasy kit (Qiagen) following manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Fragmentation and biotin labelling of each amplified cDNA was processed with the NuGen Encore Biotin Module (NuGen Inc.) and according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Gene expression data from conditionally immortalized podocytes (AB81)
|
| Data processing |
The raw data was normalized using Robust Multichip Algorithm (RMA) using CustomCDF v 18 ENTREZG
|
| |
|
| Submission date |
May 25, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Felix Eichinger |
| E-mail(s) |
eichinge@umich.edu
|
| Organization name |
The University of Michigan
|
| Department |
Division of Nephrology
|
| Street address |
1150 W. Medical Center Drive
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL19109 |
| Series (2) |
| GSE99323 |
Transcriptome-based network analysis reveals renal cell type-specific dysregulation of hypoxia-associated transcripts [AB81] |
| GSE99340 |
Transcriptome-based network analysis reveals renal cell type-specific dysregulation of hypoxia-associated transcripts |
|
