|
| Status |
Public on Jan 31, 2008 |
| Title |
RML_150_m137 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
brain mock-infected mouse 64
|
| Organism |
Mus musculus |
| Characteristics |
C57bl/6, female age 150 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from brain tissue by disruption in Trizol reagent (Invitrogen) and was further purified using RNeasy columns (Qiagen) according to the manufacturer’s instructions. A quantity of 10 µg of total RNA was used as a template for oligo dT primed reverse transcription which incorporated amino allyl -dUTP (Sigma) into the cDNA. The remaining RNA template was hydrolyzed and the cDNA product purified using the QIAquick PCR purification system (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Following Agilent protocol
|
| |
|
| Channel 2 |
| Source name |
brain RML-infected mouse 137
|
| Organism |
Mus musculus |
| Characteristics |
C57bl/6, female age 150 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from brain tissue by disruption in Trizol reagent (Invitrogen) and was further purified using RNeasy columns (Qiagen) according to the manufacturer’s instructions. Total RNA was QC'd using Agilent Bioanalyser and then processed as per Agilent array standard protocols
|
| Label |
Cy5
|
| Label protocol |
Following Agilent protocol
|
| |
|
| |
| Hybridization protocol |
Following Agilent protocol
|
| Scan protocol |
Agilent scanner and feature extraction software, standard protocol
|
| Description |
All procedures done using standard Agilent protocols
|
| Data processing |
Log ratio calculated using Agilent feature extraction software and standard procedures
|
| |
|
| Submission date |
Jan 28, 2008 |
| Last update date |
Feb 04, 2008 |
| Contact name |
stephanie booth |
| E-mail(s) |
Stephanie_Booth@phac-aspc.gc.ca
|
| Organization name |
PHAC
|
| Street address |
1015 Arlington St
|
| City |
Winnipeg |
| ZIP/Postal code |
R3E 2R3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE10310 |
Comprehensive transcriptional profiling of prion infection in mouse models reveals networks of responsive genes. |
|
