|
| Status |
Public on Jul 07, 2017 |
| Title |
colorectal cancer cell line, HCT15 |
| Sample type |
RNA |
| |
|
| Source name |
ATCC(Supplier ID CCL-225)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT15
sample type: colorectal cancer cell line
|
| Growth protocol |
Grown in medium according to supplier recommendations with added fetal bovine serum, antibiotics Streptomycin and Penicillin, along with amino acid L-glutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cell pellets using the Qiagen Allprep DNA/RNA/miRNA Universal kit, according to the manufacturer's protocol (Qiagen).
|
| Label |
biotin
|
| Label protocol |
RNA was amplified, fragmented and labelled according to the Affymetrix GeneChip HT WT User Guide.
|
| |
|
| Hybridization protocol |
Samples were hybridized onto Affymetrix Human Transcriptome 2.0 Arrays according to the manufacturer's protocol (Affymetrix).
|
| Scan protocol |
Array scanning was performed according to the manufacturer's protocol (Affymetrix).
|
| Description |
SST RMA expression values at the gene-level derived using Expression Console software
|
| Data processing |
Gene-level expression signals were obtained from raw data CEL files by the SST RMA procedure implemented in the Affymetrix Expression Console software.
affymetrix-algorithm-name = sst-rma-gene-full
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.4.1.46
|
| |
|
| Submission date |
Mar 24, 2017 |
| Last update date |
Jul 07, 2017 |
| Contact name |
Anita Sveen |
| E-mail(s) |
anita.sveen@rr-research.no
|
| Organization name |
Institute for Cancer Research, Oslo University Hospital
|
| Department |
Department of Molecular Oncology
|
| Street address |
P.O.Box 4953, Nydalen
|
| City |
Oslo |
| ZIP/Postal code |
NO-0424 |
| Country |
Norway |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE97023 |
Expression profiling of colorectal cancer cell lines. |
|
