|
| Status |
Public on Feb 20, 2008 |
| Title |
Normal Lung_GT01421 |
| Sample type |
RNA |
| |
|
| Source name |
Normal Lung Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: Male
Age at Diagnosis: 68
Cigarette Smoking Status: Never Smoked
Stage:IB
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA and following clean-up with Qiagen Rneasy Mini Kit were performed according to the manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 to 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip H133-A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene Chip Scanner.
|
| Description |
Gene expression data
|
| Data processing |
Gene expression data were processed and normalized using Bioconductor Affy package, based on the Robust Multichip Average (RMA) method5 for single-channel Affymetrix chips
|
| |
|
| Submission date |
Jan 04, 2008 |
| Last update date |
Aug 18, 2014 |
| Contact name |
Maxwell Lee |
| E-mail(s) |
leemax@mail.nih.gov
|
| Phone |
240-276-5239
|
| Organization name |
National Cancer Institute, NIH
|
| Department |
CCR
|
| Lab |
Laboratory of Human Carcinogenesis
|
| Street address |
Building 37, Room 5120
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE10072 |
Gene expression signature of cigarette smoking and its role in lung adenocarcinoma development and survival |
|
| Relations |
| Reanalyzed by |
GSE60486 |
