|
| Status |
Public on Oct 01, 2008 |
| Title |
sua5D 12th-2 |
| Sample type |
RNA |
| |
|
| Source name |
sua5D 300 generations after sporulation
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
background: dissection sproe of BY4743 MAT a
sua5 disruption
restreak on YPD plate 12 times, innoculate in 5ml YPD liquid medium
harvest at mid-log phase
|
| Biomaterial provider |
Feilong Meng, from Telomere, telomerase and cellular aging group in SIBS
|
| Treatment protocol |
innoculate in 5ml YPD liquid medium
harvest at mid-log phase
|
| Growth protocol |
innoculate in 5ml YPD liquid medium
harvest at mid-log phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
the RNeasy Mini Kit (Qiagen) and on-column DNase Digestion Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
IVT Labeling Kit Affymetrix
|
| |
|
| Hybridization protocol |
Hybridization Control Kit Affymetrix
|
| Scan protocol |
Gene array Scanner3000 Affymetrix
|
| Description |
performed following Affymetrix standard protocols
|
| Data processing |
GCOS1.2 Affymetrix
|
| |
|
| Submission date |
Dec 20, 2007 |
| Last update date |
Dec 21, 2007 |
| Contact name |
feilong meng |
| E-mail(s) |
flmeng@sibs.ac.cn
|
| Phone |
86-21-54921092
|
| Fax |
86-21-54921076
|
| Organization name |
Shanghai institutes for biology sciences
|
| Department |
SIBCB
|
| Lab |
telomere and telomerase
|
| Street address |
320 yuyang road
|
| City |
shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
|
