|
| Status |
Public on Dec 10, 2007 |
| Title |
MRC5-iPS2 iPS cells_22 |
| Sample type |
RNA |
| |
|
| Source name |
MRC5-iPS2 iPS cells
|
| Organism |
Homo sapiens |
| Characteristics |
iPS cells
|
| Treatment protocol |
Cells were washed with PBS and Rneasy kit was used to isolate total RNA
|
| Growth protocol |
hES cell, and iPS cells were cultured in hES medium containing bFGF. Somatic cells were cultured in alpha-MEM with 10% IFS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy kit was used to isolate total RNA
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
gene expresion of iPS production
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 10, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
In-Hyun Park |
| E-mail(s) |
inhyun.park@childrens.harvard.edu
|
| Organization name |
Children's Hospital Boston
|
| Department |
Hem/Onc
|
| Street address |
300 Longwood
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9832 |
Reprogramming of human somatic cells to pluripotency with defined factors |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
