|
Status |
Public on Jun 13, 2017 |
Title |
shGLP dex 0h rep1 |
Sample type |
RNA |
|
|
Source name |
shGLP dex 0h
|
Organism |
Homo sapiens |
Characteristics |
cell line: A549 treatment: 100nM dexamethasone time point: 0h knockdown: shGLP
|
Treatment protocol |
Cells were treated with 100nM dexamethasone for 24 hours
|
Growth protocol |
Cells were maintained in DMEM and hormone treated cells were plated and grown in phenol red-free medium supplemented with 5% charcoal stripped serum for 3 days prior to hormone treatment
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the TRIzol method as per the manufacturer's instructions
|
Label |
biotin
|
Label protocol |
Labeling, hybridization and scanning was performed by Epigenome Center Core Facility at USC as per their standard protocol.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 1 4849554032_B
|
Data processing |
Spatial artifacts removed with BASH, data normalized using neqc with limma in R
|
|
|
Submission date |
Feb 08, 2017 |
Last update date |
Jun 13, 2017 |
Contact name |
Dai-Ying Wu |
Organization name |
University of Southern California
|
Department |
Biochemistry
|
Lab |
Stallcup
|
Street address |
1441 Eastlake Ave, NOR 6314
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90033 |
Country |
USA |
|
|
Platform ID |
GPL6883 |
Series (1) |
GSE94646 |
Promoter context-specific positive and negative transcriptional regulation of Glucocorticoid Receptor target genes by coregulator GLP. |
|