|
| Status |
Public on Aug 04, 2008 |
| Title |
jurkat_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Jurkat cells rep 1
|
| Organism |
Homo sapiens |
| Characteristics |
Jurkat, Human T-acute lymphoblastic leukemia
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
Allowed to reach 6 X 10 5 cells/ml.
|
| Growth protocol |
RPMI-1640 (Mediatech), 10% FBS (Gibco), 2mmol/L L-glutamine (Mediatech), 100IU/ml penicillin, 100 microgram/ml streptomycin, 0.25 microgram/ml amphotericin B (all from Mediatech) at 37 degrees Celsius, 5% carbon dioxide, 95% air, and 80% relative humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
biotin
|
| Label protocol |
IVT Labeling Affymetrix
|
| |
|
| Hybridization protocol |
GeneChip Hybridization oven 640
|
| Scan protocol |
GeneChip Scanner
|
| Description |
Jurkat cells are an immortalized line of T lymphocyte cells that are used to study acute T cell leukemia. The cell line was established from peripheral blood of a human with T cell leukemia.
|
| Data processing |
MAS5.0
|
| |
|
| Submission date |
Nov 20, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nathan J. Bowen |
| E-mail(s) |
nbowen@cau.edu
|
| Phone |
4048808158
|
| Organization name |
Clark Atlanta University
|
| Department |
Center for Cancer Research and Therapeutic Development
|
| Lab |
Cancer Development and Evolution
|
| Street address |
223 James P Brawley Drive
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30314 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9648 |
Anticancer metabolites discovered by Computational Metabolomics |
|
| Relations |
| Reanalyzed by |
GSE119087 |
