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| Status |
Public on Mar 22, 2017 |
| Title |
E14.5_XX_Meioc_wild-type_RNA-seq_2 |
| Sample type |
SRA |
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| Source name |
fetal ovary
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| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: ovary
Stage: E14.5
genotype: wild-type
preclearing: none
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| Extracted molecule |
total RNA |
| Extraction protocol |
For RNA-seq experiments, total RNA, of about 1 ug, was extracted using Trizol (Invitrogen) according to manufacturer’s instructions.
For RNA-seq experiments, TruSeq Sample Preparation kit protocol was use with poly-A selection, following the manufacturer's recommendations.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
Meioc_control_RNA-seq_2
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| Data processing |
Sequenced reads were mapped to mm10 using Tophat with parameter -g1
FPKMs were calculated using Cufflinks
For RIP-seq data, counts were obtained from HTSeq and normalized via DESeq2
Genome_build: mm10
Supplementary_files_format_and_content: Cufflinks output, counts from HTSeq, or normalized counts from DESeq2
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| Submission date |
Nov 30, 2016 |
| Last update date |
Apr 08, 2024 |
| Contact name |
Maria M. Mikedis |
| E-mail(s) |
maria.mikedis@cchmc.org
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| Organization name |
Cincinnati Children's Hospital Medical Center
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| Street address |
240 Albert Sabin Way
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| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platform ID |
GPL13112 |
| Series (2) |
| GSE90702 |
Identification of the embryonic germ cell Meioc-/- transcriptome, MEIOC targets in postnatal testis, and YTHDC2 targets in postnatal testis in mouse |
| GSE96919 |
Identification of the mouse embryonic germ cell Meioc-/- transcriptome |
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| Relations |
| BioSample |
SAMN06076951 |
| SRA |
SRX2694296 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM2410933_Gm1564WT_7558_24_genes.fpkm_tracking.gz |
275.3 Kb |
(ftp)(http) |
FPKM_TRACKING |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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