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Sample GSM2410930 Query DataSets for GSM2410930
Status Public on Mar 22, 2017
Title E14.5_XX_Meioc_knock-out_RNA-seq_2
Sample type SRA
Source name fetal ovary
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: ovary
Stage: E14.5
genotype: Meioc knock-out
preclearing: none
Extracted molecule total RNA
Extraction protocol For RNA-seq experiments, total RNA, of about 1 ug, was extracted using Trizol (Invitrogen) according to manufacturer’s instructions.
For RNA-seq experiments, TruSeq Sample Preparation kit protocol was use with poly-A selection, following the manufacturer's recommendations.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Description Meioc_KO_RNA-seq_2
Data processing Sequenced reads were mapped to mm10 using Tophat with parameter -g1
FPKMs were calculated using Cufflinks
For RIP-seq data, counts were obtained from HTSeq and normalized via DESeq2
Genome_build: mm10
Supplementary_files_format_and_content: Cufflinks output, counts from HTSeq, or normalized counts from DESeq2
Submission date Nov 30, 2016
Last update date Apr 08, 2024
Contact name Maria M. Mikedis
Organization name Cincinnati Children's Hospital Medical Center
Street address 240 Albert Sabin Way
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platform ID GPL13112
Series (2)
GSE90702 Identification of the embryonic germ cell Meioc-/- transcriptome, MEIOC targets in postnatal testis, and YTHDC2 targets in postnatal testis in mouse
GSE96919 Identification of the mouse embryonic germ cell Meioc-/- transcriptome
BioSample SAMN06076954
SRA SRX2694293

Supplementary file Size Download File type/resource
GSM2410930_Gm1564KO_7757_21_genes.fpkm_tracking.gz 266.3 Kb (ftp)(http) FPKM_TRACKING
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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