|
| Status |
Public on Dec 31, 2018 |
| Title |
VR15 ESC_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
embryonic stem cell
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: Embryonic stem cell (Vasa::RFP Tg)
cell line: VR15
gender: male
cell type: embryonic stem cell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the RNeasy micro kit according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled cRNA samples were prepared using the Agilent Low Input Quick Amp Labeling Kit (one-color) (5190-2305) and RNA Spike-In Kit (one-color) (5188-5282) according to the manufacturer's instructions. Input was 100 ng of total RNA from each sample.
|
| |
|
| Hybridization protocol |
1.65ug of Cy3-labelled cRNAs from each sample were fragmented and hybridized to microarrays according to the manufacturer's instructions using the Gene Expression Hybridization kit (Agilent 5188-5242) and Agilent hybridization oven. After hybridization, microarrays were washed using the Gene Expression Wash Pack (Agilent 5188-5327).
|
| Scan protocol |
The microarrays were scanned using the Agilent DNA microarray scanner (Agilent G2505C). The spot signals were quantified using Feature Extraction software.
|
| Description |
Gene expression of mouse male ESCs (Vasa::RFP Tg).
Replicate3
|
| Data processing |
Data was normalized using 75th percentile shift and analyzed by GeneSpring GX (Agilent).
|
| |
|
| Submission date |
Nov 09, 2016 |
| Last update date |
Dec 31, 2018 |
| Contact name |
Yohei Hayashi |
| E-mail(s) |
yohei.hayashi.e2@tohoku.ac.jp
|
| Phone |
+81-22-717-8572
|
| Organization name |
Tohoku University
|
| Department |
Institute of Development, Aging and Cancer
|
| Lab |
Cell Resource Center for Biomedical Research
|
| Street address |
4-1 Seiryo-machi, Aobaku
|
| City |
Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
980-8575 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE89738 |
Characteristic energy metabolism in mouse primordial germ cells |
|
