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Sample GSM2387440 Query DataSets for GSM2387440
Status Public on Dec 31, 2018
Title VR15 ESC_rep3
Sample type RNA
Source name embryonic stem cell
Organism Mus musculus
Characteristics strain/background: Embryonic stem cell (Vasa::RFP Tg)
cell line: VR15
gender: male
cell type: embryonic stem cell
Extracted molecule total RNA
Extraction protocol Total RNA was purified using the RNeasy micro kit according to the manufacturer's instructions.
Label Cy3
Label protocol Cy3-labeled cRNA samples were prepared using the Agilent Low Input Quick Amp Labeling Kit (one-color) (5190-2305) and RNA Spike-In Kit (one-color) (5188-5282) according to the manufacturer's instructions. Input was 100 ng of total RNA from each sample.
Hybridization protocol 1.65ug of Cy3-labelled cRNAs from each sample were fragmented and hybridized to microarrays according to the manufacturer's instructions using the Gene Expression Hybridization kit (Agilent 5188-5242) and Agilent hybridization oven. After hybridization, microarrays were washed using the Gene Expression Wash Pack (Agilent 5188-5327).
Scan protocol The microarrays were scanned using the Agilent DNA microarray scanner (Agilent G2505C). The spot signals were quantified using Feature Extraction software.
Description Gene expression of mouse male ESCs (Vasa::RFP Tg).
Data processing Data was normalized using 75th percentile shift and analyzed by GeneSpring GX (Agilent).
Submission date Nov 09, 2016
Last update date Dec 31, 2018
Contact name Yohei Hayashi
Phone +81-22-717-8572
Organization name Tohoku University
Department Institute of Development, Aging and Cancer
Lab Cell Resource Center for Biomedical Research
Street address 4-1 Seiryo-machi, Aobaku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
Platform ID GPL11202
Series (1)
GSE89738 Characteristic energy metabolism in mouse primordial germ cells

Data table header descriptions
VALUE Normalized signal intensity (baseline of the data was median transformed)

Data table
GE_BrightCorner -0.31864405
DarkCorner 0.062027454
A_55_P1989846 -0.45597172
A_55_P1991598 0.07616711
A_55_P2022211 -1.3394618
A_55_P1980764 -0.61293507
A_55_P1964375 0.056317806
A_51_P128876 1.4142694
A_55_P2121042 0.0751524
A_52_P219230 0.07458782
A_51_P207591 4.1396356
A_55_P2131920 -4.926138
A_55_P2404223 -2.463981
A_55_P2101944 -0.52772236
A_52_P358860 0.8016834
A_51_P119031 0.32453156
A_51_P309854 -0.29532766
A_51_P343900 -8.031288
A_51_P234359 -4.1946993
A_51_P487813 -0.448606

Total number of rows: 39485

Table truncated, full table size 952 Kbytes.

Supplementary file Size Download File type/resource
GSM2387440_US80703200_252665518008_S02_GE1_107_Sep09_1_4_VR15c.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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