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Sample GSM2387438 Query DataSets for GSM2387438
Status Public on Dec 31, 2018
Title VR15 ESC_rep1
Sample type RNA
Source name embryonic stem cell
Organism Mus musculus
Characteristics strain/background: Embryonic stem cell (Vasa::RFP Tg)
cell line: VR15
gender: male
cell type: embryonic stem cell
Extracted molecule total RNA
Extraction protocol Total RNA was purified using the RNeasy micro kit according to the manufacturer's instructions.
Label Cy3
Label protocol Cy3-labeled cRNA samples were prepared using the Agilent Low Input Quick Amp Labeling Kit (one-color) (5190-2305) and RNA Spike-In Kit (one-color) (5188-5282) according to the manufacturer's instructions. Input was 100 ng of total RNA from each sample.
Hybridization protocol 1.65ug of Cy3-labelled cRNAs from each sample were fragmented and hybridized to microarrays according to the manufacturer's instructions using the Gene Expression Hybridization kit (Agilent 5188-5242) and Agilent hybridization oven. After hybridization, microarrays were washed using the Gene Expression Wash Pack (Agilent 5188-5327).
Scan protocol The microarrays were scanned using the Agilent DNA microarray scanner (Agilent G2505C). The spot signals were quantified using Feature Extraction software.
Description Gene expression of mouse male ESCs (Vasa::RFP Tg).
Data processing Data was normalized using 75th percentile shift and analyzed by GeneSpring GX (Agilent).
Submission date Nov 09, 2016
Last update date Dec 31, 2018
Contact name Yohei Hayashi
Phone +81-22-717-8572
Organization name Tohoku University
Department Institute of Development, Aging and Cancer
Lab Cell Resource Center for Biomedical Research
Street address 4-1 Seiryo-machi, Aobaku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
Platform ID GPL11202
Series (1)
GSE89738 Characteristic energy metabolism in mouse primordial germ cells

Data table header descriptions
VALUE Normalized signal intensity (baseline of the data was median transformed)

Data table
GE_BrightCorner -0.004683018
DarkCorner 0.2492261
A_55_P1989846 -1.1339693
A_55_P1991598 0.21162891
A_55_P2022211 -1.321713
A_55_P1980764 0.027026176
A_55_P1964375 -0.010967732
A_51_P128876 1.2978878
A_55_P2121042 0.20428133
A_52_P219230 0.20345736
A_51_P207591 4.0282083
A_55_P2131920 -0.17901587
A_55_P2404223 0.5863378
A_55_P2101944 0.3284731
A_52_P358860 0.71222115
A_51_P119031 0.30321884
A_51_P309854 -0.23920679
A_51_P343900 0.5041604
A_51_P234359 -2.6038027
A_51_P487813 -0.5919852

Total number of rows: 39485

Table truncated, full table size 947 Kbytes.

Supplementary file Size Download File type/resource
GSM2387438_US80703200_252665518008_S02_GE1_107_Sep09_1_2_VR15a.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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