|
| Status |
Public on Jan 06, 2017 |
| Title |
Nat1-null_ESC2_ 2i+LIF |
| Sample type |
RNA |
| |
|
| Source name |
Nat1-null mouse embryonic stem cells, 2i+LIF
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Nat1-null
cell type: mouse embryonic stem cell line
treatment: 2i+LIF
|
| Treatment protocol |
Mouse embryonic stem cells were treated with LIF or 2i+LIF.
|
| Growth protocol |
Standard mouse ESC medium on gelatin-coated plates at 37 ºC in a humidified incubator with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the miRNeasy Purification kit (Qiagen) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
Manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Manufacturer's protocol.
|
| Scan protocol |
Slides were scanned on the G2565BA Microarray Scanner System (Agilent).
|
| Description |
Nat1-null mouse embryonic stem cells cultured with 2i+LIF, replicate 2.
|
| Data processing |
GeneSpring GX. Signals were normalized to percentile.
|
| |
|
| Submission date |
Oct 20, 2016 |
| Last update date |
Jan 06, 2017 |
| Contact name |
Shinya Yamanaka |
| E-mail(s) |
yamanaka@cira.kyoto-u.ac.jp
|
| Phone |
81-75-366-7041
|
| Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
|
| Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10787 |
| Series (2) |
| GSE89007 |
Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells [expression] |
| GSE89012 |
Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells |
|
