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Sample GSM2357064 Query DataSets for GSM2357064
Status Public on Jan 06, 2017
Title Nat1-null_ESC2_ 2i+LIF
Sample type RNA
Source name Nat1-null mouse embryonic stem cells, 2i+LIF
Organism Mus musculus
Characteristics genotype/variation: Nat1-null
cell type: mouse embryonic stem cell line
treatment: 2i+LIF
Treatment protocol Mouse embryonic stem cells were treated with LIF or 2i+LIF.
Growth protocol Standard mouse ESC medium on gelatin-coated plates at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the miRNeasy Purification kit (Qiagen) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Manufacturer's protocol.
Hybridization protocol Manufacturer's protocol.
Scan protocol Slides were scanned on the G2565BA Microarray Scanner System (Agilent).
Description Nat1-null mouse embryonic stem cells cultured with 2i+LIF, replicate 2.
Data processing GeneSpring GX. Signals were normalized to percentile.
Submission date Oct 20, 2016
Last update date Jan 06, 2017
Contact name Shinya Yamanaka
Phone 81-75-366-7041
Organization name Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
Street address 53 Kawahara-machi, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
Platform ID GPL10787
Series (2)
GSE89007 Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells [expression]
GSE89012 Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 5.3226757
DarkCorner -6.545888
A_55_P2051983 -6.5441413
A_52_P169082 -1.2853546
A_30_P01028193 -4.7827826
A_52_P237997 -6.526437
A_51_P414243 1.6713734
A_55_P2136348 -6.5172663
A_51_P108228 -5.002431
A_30_P01033363 -5.252099
A_55_P2049737 -6.506896
A_30_P01024440 -0.52122974
A_30_P01025554 3.3259773
A_30_P01031558 -4.5253263
A_30_P01030675 -6.499371
A_51_P328014 3.6054096
A_30_P01019108 -0.5290413
A_55_P2056220 2.260439
A_55_P1985764 7.5925684
A_52_P108321 -0.4934759

Total number of rows: 55821

Table truncated, full table size 1333 Kbytes.

Supplementary file Size Download File type/resource
GSM2357064_11._NAT1_KO_2i_2.txt.gz 3.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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