|
| Status |
Public on Nov 01, 2016 |
| Title |
203_BA25 |
| Sample type |
genomic |
| |
|
| Source name |
BA25
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: cortex, Brodmann area 25
age: 85
groupid: MDD suicide case
gender: F
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated from ~50 mg of each dissected brain region using a standard phenol-chloroform extraction method, and tested for degradation and purity before analysis.
|
| Label |
Cy3 and Cy5
|
| Label protocol |
500 ng DNA from each sample was sodium bisulfite-treated using the EZ-96 DNA Methylation-Gol Kit (Zymo Research) according to the manufacturer's standard protocol.
|
| |
|
| Hybridization protocol |
Samples were processed according to the Illumina Infinium HumanMethylation450K BeadChip (Illumina) protocol.
|
| Scan protocol |
Samples were assessed using the Illumina Infinium HumanMethylation450K BeadChip (Illumina) using a Illumina iScan System (Illumina).
|
| Description |
9283265107_R04C02
|
| Data processing |
Illumina Genome Studio software was used to extract the raw signal intensities of each probe (without background correction or normalization). Probes containing a SNP with minor allele frequency (MAF) >5% within 10bp of the single base extension site based on Illumina's database and probes identified by Chen et al. (Epigenetics 2013, 8:203-209) were removed. Data was then pre-processed in the R package wateRmelon using the pfilter and dasen functions.
|
| |
|
| Submission date |
Oct 18, 2016 |
| Last update date |
Nov 01, 2016 |
| Contact name |
Therese M Murphy |
| E-mail(s) |
t.murphy@exeter.ac.uk
|
| Organization name |
University of Exeter
|
| Department |
University of Exeter Medical School
|
| Street address |
RILD building
|
| City |
Exeter |
| ZIP/Postal code |
EX2 5DW |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE88890 |
Major depression MDD suicide brain study |
|
