|
| Status |
Public on Jan 31, 2017 |
| Title |
LN10_SNB-_N |
| Sample type |
RNA |
| |
|
| Source name |
biopsy from sentinel lymph node
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: sentinel lymph node
patient id: LN10
disease: Melanoma
snb status: negative
progression status at 5 years follow up: not progressing (N)
stage after surgery: IB
primary tumor classification: T2b
primary tumor localization: Foot
gender: female
age (years): 51
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with RecoverAll Total Nucleic Acid Isolation Kit (Ambion) according to the protocol provided by the manufacturer.
|
| Label |
Cy3
|
| Label protocol |
Total RNA from each sample was labeled according to the manufacturers recommendations.
|
| |
|
| Hybridization protocol |
Standard Agilent hybridization protocol
|
| Scan protocol |
Standard Agilent scanning protocol with Agilent SureScan microarray scanner (G2505C).
|
| Data processing |
Scanned images were processed with Agilent Feature Extraction v10.7 with default parameters to obtain raw data. Raw data were imported in R and processed using the limma package. After background correction with the normexp method, raw data were normalized with quantile method and log2 transformed.
|
| |
|
| Submission date |
Oct 13, 2016 |
| Last update date |
Jan 31, 2017 |
| Contact name |
Matteo Dugo |
| E-mail(s) |
dugo.matteo@hsr.it
|
| Organization name |
IRCCS Ospedale San Raffaele
|
| Department |
Department of Medical Oncology
|
| Street address |
via Olgettina 60
|
| City |
Milan |
| ZIP/Postal code |
20132 |
| Country |
Italy |
| |
|
| Platform ID |
GPL15159 |
| Series (1) |
| GSE88727 |
microRNA expression profiles of tumor-positive and tumor-negative sentinel lymph node biopsies from cutaneous melanoma patients |
|
