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Sample GSM2335243 Query DataSets for GSM2335243
Status Public on Apr 04, 2017
Title 50-MDD-L3-1
Sample type RNA
 
Source name dorsolateral prefrontal cortex
Organism Homo sapiens
Characteristics tissue: dorsolateral prefrontal cortex
cell type: pyramidal neuron cell
shape: characteristic triangular shape and prominent apical dendrite
genotype: Major Depressive Disorder
dsm-iv diagnoses: MDD, recurrent
Treatment protocol An independent committee of experienced research clinicians made consensus DSM-IV diagnoses for each subject using information obtained from medical records and structured diagnostic interviews conducted with the decedent’s family members.
Growth protocol Brain specimens were obtained during autopsies conducted at the Allegheny County Office of the Medical Examiner (Pittsburgh, PA) after consent was obtained from the next-of-kin.
Extracted molecule total RNA
Extraction protocol For each sample, RNA was extracted using the QIAGEN® RNeasy Plus Micro kit (QIAGEN, Valencia).
Label Encore Biotin module (Nugen)
Label protocol Labeled using the Encore Biotin module (Nugen).
 
Hybridization protocol CDNA was synthesized and amplified using the Ovation Pico WTA System.
Scan protocol Loaded on an Affymetrix GeneChip® U219.
Description Gene expression data for subject 50, diagnosis MDD, layer 3, replicate 1
Data processing Expression intensities were extracted from Affymetrix Expression Console and normalized using Robust Multi-array Average Express.
 
Submission date Oct 04, 2016
Last update date Apr 04, 2017
Contact name David Lewis
E-mail(s) lewisda@upmc.edu
Organization name University of Pittsburgh
Department Psychiatry and Neuroscience
Street address 3811 O'Hara St
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
 
Platform ID GPL13667
Series (1)
GSE87610 Gene expression of L3 and L5 pyramidal neurons in the DLPFC comparing schizophrenia from bipolar major depressive disorders and unaffected subjects.

Data table header descriptions
ID_REF
VALUE Background corrected, log2 transformed and then quantile normalized gene expression intensity.

Data table
ID_REF VALUE
AFFX-DapX-5_at 2.45694
AFFX-DapX-M_at 2.502289
AFFX-DapX-3_at 2.329885
AFFX-LysX-5_at 1.834237
AFFX-LysX-M_at 2.213631
AFFX-LysX-3_at 2.158607
AFFX-PheX-5_at 1.824622
AFFX-PheX-M_at 2.636173
AFFX-PheX-3_at 2.523691
AFFX-ThrX-5_at 2.352163
AFFX-ThrX-M_at 2.312839
AFFX-ThrX-3_at 2.972013
AFFX-TrpnX-5_at 2.052997
AFFX-TrpnX-M_at 2.215986
AFFX-TrpnX-3_at 2.297094
AFFX-r2-Ec-bioB-5_at 7.790367
AFFX-r2-Ec-bioB-M_at 7.924616
AFFX-r2-Ec-bioB-3_at 7.874006
AFFX-r2-Ec-bioC-5_at 9.167496
AFFX-r2-Ec-bioC-3_at 8.75514

Total number of rows: 49386

Table truncated, full table size 1081 Kbytes.




Supplementary file Size Download File type/resource
GSM2335243_5500994192207072414569_F04.cel.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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