|
| Status |
Public on May 07, 2008 |
| Title |
GUYT2_50362 |
| Sample type |
RNA |
| |
|
| Source name |
breast
|
| Organism |
Homo sapiens |
| Characteristics |
samplename:GUYT2_50362
series:GUYT2
age:50
grade:2
size:1.2
er:1
pgr:1
node:0
t.rfs:2946
e.rfs:0
t.dmfs:2946
e.dmfs:0
treatment:tamoxifen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc).
|
| Label |
biotin
|
| Label protocol |
Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures.
|
| Scan protocol |
standard Affymetrix procedures.
|
| Description |
Tumor samples from Tamoxifen-only treated primary breast cancer patients
|
| Data processing |
RMA Bioconductor
|
| |
|
| Submission date |
Sep 28, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Benjamin Haibe-Kains |
| E-mail(s) |
benjamin.haibe.kains@utoronto.ca
|
| Phone |
+14165818626
|
| Organization name |
Princess Margaret Cancer Centre
|
| Department |
Princess Margaret Research
|
| Lab |
Bioinformatics and Computational Genomics
|
| Street address |
610 University Avenue
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9195 |
Predicting prognosis using molecular profiling in estrogen receptor-positive breast cancer treated with tamoxifen |
|
| Relations |
| Reanalyzed by |
GSE119087 |
