|
Status |
Public on May 07, 2008 |
Title |
LT-HSC_Control_Rep2 |
Sample type |
RNA |
|
|
Source name |
Long-term hematopoietic stem cells, control
|
Organism |
Mus musculus |
Characteristics |
Mouse model: MxCre-.Pbx1f/f on a C57B/6 background Age: 7.5 wks old Tissue: Bone marrow LT-HSC (Lin-cKit+Sca1+CD34-CD135-)
|
Treatment protocol |
7 poly(I:C) injections every other day, last injection 4wks before sacrifice
|
Extracted molecule |
polyA RNA |
Extraction protocol |
RNA from sorted cells was purified using Trizol and subjected to two rounds of amplification.
|
Label |
biotin
|
Label protocol |
GeneChip® IVT Labeling Kit (Affymetrix)
|
|
|
Hybridization protocol |
GeneChip® Hybridization Control Kit (Affymetrix)
|
Scan protocol |
Arrays were scanned with a Gene Chip Scanner 3000 (Affymetrix) running GCOS 1.1.1 software.
|
Description |
See GeneChip® Two-Cycle Target Labeling Assay protol (Affymetrix)
|
Data processing |
Scanned data were exported to dChip software for normalization, statistical analysis and heat mapping. A perfect-match/mismatch (PM/MM) model was used for the calculation of expression values.
|
|
|
Submission date |
Sep 27, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Francesca Ficara |
E-mail(s) |
francesca.ficara@humanitasresearch.it
|
Organization name |
CNR/Istituto Clinico Humanitas
|
Street address |
via Manzoni 56
|
City |
Rozzano |
State/province |
MI |
ZIP/Postal code |
20089 |
Country |
Italy |
|
|
Platform ID |
GPL1261 |
Series (3) |
GSE9188 |
Differentially regulated genes in LT-HSC from control or Pbx1-null mice |
GSE9189 |
Differentially regulated genes in normal LT-HSC vs ST-HSC |
GSE9198 |
Long-term hematopoietic stem cells and the proto-oncogene Pbx1 |
|
Relations |
Reanalyzed by |
GSE119085 |